New algebraic model of DNA hybridization to measure cellular behavior

March 08, 2016

However, the researchers' initial work involved not experiments, but, rather, the creation of mathematical models to predict "DNA-cDNA duplex formation." They developed an algebraic computation that allowed them to model arbitrary DNA-cDNA duplex formation, and, with it, measurements of cellular behavior. Specifically, they assigned to various chemical properties of DNA strands different algebraic values (e.g., "K," "X," "Y"). They then ran a series of computations that resulted in expressing how "matches" or "mismatches" among various strands of DNA can be characterized by the input algebraic variables. These computations could then be used directly to design the most accurate biotechnology for measuring cellular behavior.

To confirm the validity of these algebraic models, the researchers conducted laboratory experiments involving the hybridization of DNA sequences. These results largely confirmed those predicted by the mathematical models-the DNA sequences in the laboratory matched up in most instances in ways the models forecast.

Source: New York University